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MULTI DRUG RESISTANCE PATTERNS OF LISTERIA SPECIES IN FROZEN AND LIVE CHICKEN SOLD

ABSTRACT

This study carried out aimed at determine the multi drug resistance patterns of Listeria speciesin frozen and live chicken sold in Owerri. Isolated Listeria spp. where identified using biochemical and bacteriological identification scheme. The antimicrobial susceptibility was tested for each isolates. The following antibiotics tested using Bauer–Kirby technique (Anon. 1997) are, 10 μg ampicillin (A10), 30 μg chloramphenicol (C30), 15 μg erythromycin (E15), 10 μg gentamicin (CN10), 10 units penicillin G (P10), 10 μg streptomycin (S10), 30 μg tetracycline (TE30) or 30 μg vancomycin (VA30). After inoculation (organisms and antibiotics) and incubation of the plates with the antibiotics, the diameter (in mm) of the zone around each disc was measured and interpreted in accordance with the National Committee for Clinical Laboratory Standards (NCCLS) guidelines. Form the study, Chloramphenicol, Gentamycin, Vanconmycin and Erthromycin were 100% sensitive. Streptomycin and Penicillin were 75% sensitive and  Tetracyclin and Ampicillin showed 50% sensitive.

MULTI DRUG RESISTANCE PATTERNS OF LISTERIA SPECIES IN FROZEN AND LIVE CHICKEN SOLD

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MULTI DRUG RESISTANCE PATTERNS OF LISTERIA SPECIES IN FROZEN AND LIVE CHICKEN SOLD

ABSTRACT

This study carried out aimed at determine the multi drug resistance patterns of Listeria speciesin frozen and live chicken sold in Owerri. Isolated Listeria spp. where identified using biochemical and bacteriological identification scheme. The antimicrobial susceptibility was tested for each isolates. The following antibiotics tested using Bauer–Kirby technique (Anon. 1997) are, 10 μg ampicillin (A10), 30 μg chloramphenicol (C30), 15 μg erythromycin (E15), 10 μg gentamicin (CN10), 10 units penicillin G (P10), 10 μg streptomycin (S10), 30 μg tetracycline (TE30) or 30 μg vancomycin (VA30). After inoculation (organisms and antibiotics) and incubation of the plates with the antibiotics, the diameter (in mm) of the zone around each disc was measured and interpreted in accordance with the National Committee for Clinical Laboratory Standards (NCCLS) guidelines. Form the study, Chloramphenicol, Gentamycin, Vanconmycin and Erthromycin were 100% sensitive. Streptomycin and Penicillin were 75% sensitive and  Tetracyclin and Ampicillin showed 50% sensitive.

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