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MEASUREMENT OF COMPLETE BLOOD COUNT (CBC) IN ALCOHOL CONSUMER – KHARTOUM 2014
ABSTRACT
This is a prospective case control study to investigate the effect of alcohol consumption on complete blood count (CBC) of alcohol consumers in Khartoum State from April to July 2014.The participants were 80 apparently healthy adult males; 50 of them are alcohol consumers and 30 are non-alcoholic (control).Their age was (41 ±7.3years).A questionnaire was constructed to obtain information about the participants after an informed verbal consent from all the participants. Ethical approval was obtained from the College of Medical Laboratory Sciences-SUST. The educational level of the subjects was 74% primary, 16% secondary and 10% had higher education. 84% of the alcoholic’s participants use local drinks while the rest16% drink imported alcohol. The duration of alcohol consumption was 5-20years. Blood was collected from all participants into EDTA containers then CBC was carried out immediately after collection; using an automated hematological analyzer (Sysmex KX-21N). Data obtained was analyzed using software program statistical package of social science (SPSS.V.11.5).The results obtained from alcoholic compared with the control were insignificantly different (P≤0.05) with regard to erythrocytes count (4.53±0.6),hemoglobin level (14.28±0.23) hematocrit(39.26±0.57), mean cell volume(86.75±1.54), mean cell hemoglobin (29.46±0.33),total leucocytes count (6.33±1.7), monocytes% (4.3±0.3), eosinophil%(1.88±0.1), basophil %(0.48±0.08) and platelet count(251.4±5.3).
List of content
Subject Page
Dedication ii
Acknowledgment iii
Abstract iv
List of abbreviation vi
List of tables vii
List of figure vii
Content ix
Chapter One
Introduction and literature review
1.1 Introduction 1
1.2 literature review 2
1.2.1 Blood Components and Functions: 2
1.2.2 Functions of blood 2
1.2.3 Hematopoiesis 4
1.2.3.1Erythropoiesis 4
1.2.3.2 Granulopoiesis 5
1.2.3.3 Megakaryocytopoiesis 5
1.2.4 Hematological abnormalities in alcoholism: 6
1.2.4.1 Red blood cells: 6
1.2.4.2 Leukocytes: 7
1.2.4.4 Lymphocytes: 7
1.2.4.5 Monocytes: 8
1.2.4.3 Neutrophils: 9
1.2.4.6 Eosinophil’s: 10
1.2.4.7 Basophils 10
1.2.4.8 Thrombocytes 10
1.2.5.1 Anemia 11
1.2.5.2 Alcoholic disorder 13
1.2.5.3 Signs and symptoms 13
1.2.5.4Hematogical markers of alcoholism 14
1.2.5.5State Markers 14
1.2.5.6 Other Tools for Alcoholism check 15
1.2.4 Previous study 16
1.3 Rational 17
1.4 Objectives 18
1.4.1 General Objective 18
1.4.2 Specific Objectives 18
Chapter Two
Material and Methods
2.1 Study design 19
2.2 Study population 19
2.2.1 Inclusion Criteria 19
2.2.2 Exclusion Criteria 19
2.3 Sample Size 19
2.5 blood collection 19
2.5 Tools of Data Collection: 20
2.6 Principle of hematology analyzers Sysmex (KX2-1N) 20
2.6.1 Procedure of Sysmex KX-21N 21
2.6.2 Reagents and Materials 22
2.7 Preparation of thin Blood Film 22
2.8 Ethical Consideration 23
2.9 Data Presentation 23
Chapter Three
Results
3.1. Characteristics of the study population: 24
3.2 Laboratory Data 28
Chapter Four
Discussion, conclusion and recommendation
4.1 Discussion: 34
4.2. Conclusion: 35
4.3. Recommendation: 36
References 37
Appendix–1 41
Appendix–2 42
Appendix-3 43
Appendix-4 44
Appendix-5 45
Appendix-6 46
Appendix-7 47
Appendix-8 48
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