MOLECULAR DETECTION OF HEPATITIS B VIRUS GENOTYPES AMONG HUMAN IMMUNODEFICIENCY VIRUS PATIENTS IN RIVERS STATE NIGERIA

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MOLECULAR DETECTION OF HEPATITIS B VIRUS GENOTYPES AMONG HUMAN IMMUNODEFICIENCY VIRUS PATIENTS IN RIVERS STATE NIGERIA

ABSTRACT

Hepatitis B virus (HBV) and Human immunodeficiency virus (HIV) share common routes of blood-borne transmission. There are Ten genotypes from (A-I) of HBV genome, have been identified, HBV genotypes have different geographical distribution and regarding to disease progression and response of antiviral treatment and prognosis. The objective of this study was molecular detection of HBV genotypes among HIV patients in Rivers state. A total of 92 subjects (n=92) were included in this observational descriptive cross sectional study. The subjects were confirmed as HIV-positive by an Enzyme Linked Immunosorbent assays ELISA assay, ages ranged from 30 to 40 years, from both sexes. Serum samples were collected from the participants, and tested for HBsAg by a capture ELISA assay and for HBV DNA and genotypes by Nested Multiplexes PCR out of the 92 participants who took part in the study, 45 (48.9%) were positive for HBsAg and HBV DNA, the common genotypes 26 (57.7%) were genotype E and 19 (42.2%) were genotype D. From 45 positive patients 12 (26.6%) were coinfection genotype E and D. From the above findings we concluded that, there is a high percentage of HBV/HIV coinfection in the Nigeria.

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