MOLECULAR DETECTION OF EPSTEIN BARR VIRUS IN BREAST CANCER TISSUE BIOPSIES FROM SUDANESE WOMEN IN RIVERS STATE DURING 2015-2016

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MOLECULAR DETECTION OF EPSTEIN BARR VIRUS IN BREAST CANCER TISSUE BIOPSIES FROM SUDANESE WOMEN IN RIVERS STATE DURING 2015-2016

Abstract

A retrospective cross sectional molecular analysis for detection of epstein Barr virus on 51 breast cancer biopsies (paraffin embedded), previously diagnosed as breast cancer by histological examination, the samples were collected from the Military Hospital. This study was performed during the period from March to June 2017. According to their age, degree of differentiation, and type of cancer the women were divided into groups.

The specimens were treated by xylene and the paraffin removed, then the DNA was extracted followed by the detection of EBV LMP-1gene and EBV EBNA-4 gene using multiplex PCR. Out of total 51 patients, 5(9.8%) were positive for EBV, results were compared with the positive control for EBV; while 46(90.1%) were negative.

In this study EBV was only detected in invasive ductal carcinoma but not in infiltrative lobular, ductal carcinoma in situ, and mucinous carcinoma. It is also concluded that EBV LMP-1gene was only detected. There was no significant association between virus detection and type of carcinoma and type of cell differentiation. The virus was detected in age group 36-45 and 66-75 year

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