ATTENTION:
BEFORE YOU READ THE ABSTRACT OR CHAPTER ONE OF THE PROJECT TOPICS BELOW, PLEASE READ THE INFORMATION BELOW.THANK YOU!
INFORMATION:
YOU CAN GET THE COMPLETE PROJECT OF THE TOPIC BELOW. THE FULL PROJECT COST N5,000 ONLY. THE FULL INFORMATION ON HOW TO PAY AND GET THE COMPLETE PROJECT IS AT THE BOTTOM OF THIS PAGE. OR
YOU CAN CALL: 08068231953, 08137701720
WHATSAPP US ON: 08137701720
ANTIBACTERIAL ACTIVITY OF HONEY ON STREPTOCOCCUS PYOGENES, ESCHERICHIA COLI AND STAPHYLOCOCCUS AUREUS ISOLATED FROM BURN WOUNDS OF PATIENTS ATTENDING ST. MARY’S HOSPITAL LACOR
Abstract
The growing threat and spread of antibiotic resistance by a wide range of common pathogens has led to increased investigations into traditional medicinal plants as alternatives. Honey has been used as a medicine since ancient times in many cultures and is still used in ‘folk medicine’. The moist environment of chronic wounds is an ideal growth medium for bacteria thereby causing delay in healing. Honey is commonly used as a topical antibacterial agent in most cases to complement the conventional antibiotics treatment. A cross-sectional study was carried out to determine the invitro antibacterial activity of honey on some wound bacteria isolates. Fifty (50) swab samples were obtained from wounds of patients who consented to participate in the study. The specimens were streak inoculated onto MacConkey agar, blood agar, Mannitol salt agar and nutrient agar. Cultures were incubated at 37°C for 18 to 24 hours. After incubation, bacterial isolates were identified by colony morphology, Gram staining technique and biochemical tests. Results from the study revealed that Staphylococcus aureus was the most prevalent bacteria isolated 28 (38.4%), followed by Streptococcus pyogenes 25 (34.2%) and Escherichia coli 20 (27.4%). The Minimum inhibitory concentration of honey against the wound isolates shows Escherichia coli with the highest MIC (0.25 g/ml). This present study has demonstrated the antibacterial activity of honey on wound isolates.
TABLE OF CONTENTS
DECLARATION
ACKNOWLEDGEMENT
ABBREVIATIONS
ABSTRACT
CHAPTER ONE
1.0 INTRODUCTION
1.1 Background
1.2 Problem statement
1.3 Justification and significance
1.4 Aims and objectives.
CHAPTER TWO
2.0 LITERATURE REVIEW
2.1 Honey
2.1.1 The Contents of Honey
2.1.2 Classification of honey
2.1.2.1 Classification according to viscosity
2.1.2.2 Classification according to origin
2.1.2.3 Classification according to source
2.1.3 Preservation of honey
2.1.4 Nature of the Antimicrobial Activity of Honey
2.2 Burnt Wound Infection
2.2.1 Skin: Overview
2.2.2. Skin Related Infection
2.2.3 Wound Infection and Inflammation
2.2.4 Burnt Wound Infection
2.2.5 Pathogens Associated with Burn Wound Infection
CHAPTER THREE
3.0 METHODOLOGY
3.1 Research design
3.2 Research area
3.3 Research population
3.4.0 Materials and Methods
3.4.1 Sample Collection
3.4.2 Preparation of honey Sample
3.4.3 Isolation of the test organisms (bacterial isolates)
3.4.4 Standardization of inocula
3.5.1 Gram staining
3.6.1 Coagulase Test
3.6.2 Catalase test
3.6.3 Indole test
3.7 Identification of the Pathogens
3.8 Antibacterial activity of Honey
3.9 Determination of minimum inhibitory concentration (MIC)
3.10 Statistical analysis
3.11 Ethical consideration
CHAPTER FOUR
4.0 RESULTS
CHAPTER FIVE
5.0 DISCUSSION
CHAPTER SIX
6.1 Conclusions
6.2 Recommendations
REFERENCES
HOW TO RECEIVE PROJECT MATERICAL(S)
After paying the appropriate amount (#5,000) into our bank Account below, send the following information to
08068231953 or 08168759420
(1) Your project topics
(2) Email Address
(3) Payment Name
(4) Teller Number
We will send your material(s) after we receive bank alert
BANK ACCOUNTS
Account Name: AMUTAH DANIEL CHUKWUDI
Account Number: 0046579864
Bank: GTBank.
OR
Account Name: AMUTAH DANIEL CHUKWUDI
Account Number: 3139283609
Bank: FIRST BANK
FOR MORE INFORMATION, CALL:
08068231953 or 08168759420
http://graduateprojects.com.ng/