MOLECULAR DETECTION OF METRONIDAZOLE RESISTANT GENES (RDXA AND FRXA) OF HELICOBACTER PYLORI ISOLATED FROM STOOL SPECIMEN AT ALINJAZ DIAGNOSTIC CENTER IN KHARTOUM STATE

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MOLECULAR DETECTION OF METRONIDAZOLE RESISTANT GENES (RDXA AND FRXA) OF HELICOBACTER PYLORI ISOLATED FROM STOOL SPECIMEN AT ALINJAZ DIAGNOSTIC CENTER IN KHARTOUM STATE

Abstract

The bacterium H.pylori is a fastidious, microaerophilic spiral Gram negative microorganism. This study aimed for molecular detection of metronidazole resistance genes (RdxA and FrxA) inH. pylori isolated from stool specimens in Elinjaz Diagnostic Center, in Khartoum State. A total of 65 samples were collected in this study, and bacteria were identified by the immune chromatography test (ICT), chemical method (Guanidine) was used for DNA extraction, and multiplex PCR was conducted to detect metronidazole resistance genes (RdxA and FrxA) in H.pylori isolates.RdxA and FrxA genes were not detected in the isolates.

As conclusion there were no detected resistance genes to metronidazole in H. pylori in this study.

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